New Strategy Discovered To Potentially Reduce Fatigue After COVID-19 Vaccination

According to a new study, switching the administration of mRNA-based COVID-19 vaccines from intramuscular to subcutaneous injection could ease adverse post-vaccination effects, such as fatigue.

Mouse experiments suggest a switch from intramuscular to subcutaneous injection may ease fatigue.

Currently, mRNA-based COVID-19 vaccines are injected deep into the muscles, which is called intramuscular injection. An alternative type of shot is called subcutaneous injection, where a short needle can inject medication (in this case a COVID vaccine) in the tissue between the skin and the muscle. In experiments done on mice, researchers found that subcutaneous injection of the COVID-19 mRNA vaccines may reduce the adverse post-vaccination effects, such as fatigue, while still providing similar immune-system responses.

Despite their high efficacy against SARS-CoV-2, mRNA-based COVID-19 vaccines are associated with adverse post-vaccination effects, such as fatigue. How can this be avoided?

In a new study publishing today (May 31st, 2022) in the open-access journal PLOS Biology, Ayesa Syenina of the Duke–NUS Medical School in Singapore and colleagues report that a new analysis of blood samples from people vaccinated for COVID-19 has identified distinct molecular characteristics linked to an increased likelihood of post-vaccination fatigue. Additionally, experiments in mice suggest that switching the vaccine injection strategy could potentially ease such adverse effects.

Adverse post-vaccination effects may influence people’s willingness to get vaccinated or receive a booster dose, hampering efforts to reduce the spread and severity of COVID-19. However, the molecular underpinnings of adverse post-vaccination effects have been unclear.

To improve understanding, Syenina and colleagues analyzed blood samples from 175 healthcare workers who received BNT162b2, the Pfizer-BioNTech COVID-19 vaccine. Specifically, they used the blood samples to analyze a snapshot of each participant’s gene expression, or which genes are turned on or off.

This analysis revealed that people who experienced moderately severe fatigue after vaccination were more likely to have higher baseline expression of genes related to the activity of T cells and natural killer cells—two key cell types in the human immune system.

Researchers point to potential strategy to reduce fatigue after COVID-19 vaccination. Credit: Christine Tham (CC BY 4.0)

The researchers also tested two different vaccination injection strategies in mice. Some mice received BNT162b2 through intramuscular injection, the current method used for human patients, in which the vaccine is injected into the muscles. Other mice received a subcutaneous injection, in which the vaccine is injected into tissue just under the skin.

After vaccination, compared to mice that received intramuscular vaccination, mice that received subcutaneous vaccination showed immune-system responses that are in line with a lower likelihood of adverse effects such as fatigue. However, subcutaneous injection did not appear to compromise the protective effects of vaccination.

Further research will be needed to build on these findings and explore their clinical significance. Still, they boost understanding of post-vaccination fatigue and offer a potential strategy to reduce its likelihood.

Coauthor Eng Eong Ooi adds, “This study provides a first insight into the molecular basis of a side effect that many have experienced following mRNA vaccination. We hope that this finding would spur more studies to fully understand the underpinning mechanisms behind vaccine-associated side effects and collectively contribute to developing even more tolerable vaccines.”

Reference: “Adverse effects following anti–COVID-19 vaccination with mRNA-based BNT162b2 are alleviated by altering the route of administration and correlate with baseline enrichment of T and NK cell genes” 31 May 2022, PLOS Biology.
DOI: 10.1371/journal.pbio.3001643

Funding: This study was supported by the National Medical Research Council (NMRC) Open Fund-Large Collaborative Grant (OFLCG19May-0034) and Senior Clinician-Scientist Award (MOH-000135-00) to E.E.O, and the Open Fund-Young Investigator Research Grant (MOH-OFIRG18nov-0004) to R.D.A. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.