Biology

Scientists Show Transmission of Epigenetic Memory Across Multiple Generations

Advanced Genetic Engineering Illustration

A new research study reveals how a common type of epigenetic modification can be transmitted via sperm not only from parents to offspring, but to the next generation (“grandoffspring”) as well.

Changing the epigenetic marks on chromosomes results in altered gene expression in offspring and in grandoffspring, demonstrating ‘transgenerational epigenetic inheritance.’

Without changing the genetic code in the DNA, epigenetic modifications can alter how genes are expressed, affecting an organism’s health and development. It was once a radical idea that such changes in gene expression can be inherited. Now there is a growing body of evidence behind it, but the mechanisms involved are still poorly understood.

Scientists at the University of California, Santa Cruz show in a new study how a common type of epigenetic modification can be transmitted via sperm not only from parents to offspring, but to the next generation (“grandoffspring”) as well. This is called “transgenerational epigenetic inheritance.”  It may explain how a person’s health and development could be influenced by the experiences of his or her parents and grandparents.

Published the week of September 26 in the Proceedings of the National Academy of Sciences (PNAS), the study focused on a particular modification of a histone protein that changes the way DNA is packaged in the chromosomes. This widely studied epigenetic mark (called H3K27me3) is known to turn off or “repress” the affected genes. It is found in all multicellular animals—from humans to the nematode worm C. elegans used in this research.

“These results establish a cause-and-effect relationship between sperm-transmitted histone marks and gene expression and development in offspring and grandoffspring,” said corresponding author Susan Strome. She is professor emerita of molecular, cell and developmental biology at UC Santa Cruz.

Histones are the primary proteins involved in the packaging of DNA in the chromosomes. The epigenetic mark known as H3K27me3 refers to methylation of a certain amino acid in the histone H3. This results in the DNA being more densely packaged, making the genes in that region less accessible for activation.

In a study of epigenetic inheritance, researchers created embryos of the worm C. elegans that inherited egg chromosomes properly packaged with the epigenetic mark H3K27me3 and sperm chromosomes lacking the mark. The one-cell embryo on the left inherited the pink chromosomes from the egg and the green chromosomes from the sperm, the colors showing the presence or absence of H3K27me3. The two-cell embryo on the right shows the egg and sperm chromosomes united in each nucleus. Credit: Photo by Laura Gaydos

In the recent work, this histone mark was selectively stripped from the chromosomes of C. elegans sperm, which were then used to fertilize eggs with fully marked chromosomes. In the resulting offspring, the scientists observed abnormal gene expression patterns, with genes on the paternal chromosomes (inherited from the sperm) turned on or “upregulated” in the absence of the repressive epigenetic mark.

This resulted in tissues turning on genes they would not normally express. For instance, germline tissue (which produces eggs and sperm) turned on genes normally expressed in neurons.

“In all the tissues we analyzed, genes were aberrantly expressed, but different genes were turned up in different tissues, demonstrating that the tissue context determined which genes were upregulated,” Strome said.

Analysis of the chromosomes in the offspring’s germline tissue showed that the upregulated genes still lacked the repressive histone mark, while the mark had been restored on the genes that were not upregulated.

“In the germline of the offspring, some genes were aberrantly turned on and stayed in the state lacking the repressive mark, while the rest of the genome regained the mark, and that pattern was passed on to the grandoffspring,” Strome explained. “We speculate that if this pattern of DNA packaging is maintained in the germline, it could potentially be passed on for numerous generations.”

In the grandoffspring, the investigators observed a range of developmental effects, including some worms that were completely sterile. This mix of outcomes is due to how chromosomes get distributed during the cell divisions that produce sperm and eggs, resulting in many different combinations of chromosomes that can be passed on to the next generation.

Researchers in Strome’s lab have been studying epigenetic inheritance in C. elegans for years, and she said this paper represents the culmination of their work in this area. She noted that other scientists researching mammalian cells in culture have reported results very similar to her lab’s findings in worms, although those studies did not show transmission across multiple generations.

“This looks like a conserved feature of gene expression and development in animals, not just a weird worm-specific phenomenon,” she said. “We can do amazing genetic experiments in C. elegans that can’t be done in humans, and the results of our experiments in worms can have broad implications in other organisms.”

Reference: “Sperm-inherited H3K27me3 epialleles are transmitted transgenerationally in cis” Kiyomi Raye Kaneshiro, Thea A. Egelhofer, Andreas Rechtsteiner, Chad Cockrum and Susan Strome, 26 September 2022, Proceedings of the National Academy of Sciences.
DOI: 10.1073/pnas.2209471119

The co-first authors of the paper are Kiyomi Kaneshiro, who worked on the study as a graduate student in Strome’s lab and is currently a postdoctoral researcher at the Buck Institute for Research on Aging, and UCSC research associate Thea Egelhofer. The coauthors also include bioinformaticist Andreas Rechtsteiner and graduate student Chad Cockrum (now at IDEXX Laboratories). This work was supported by the National Institutes of Health.

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  • This sounds very much like the discredited 19th Century Lamarkism, which led to the disastrous Russian Lysenkoism.

    I'm reminded of Carl Sagan's caution that "Extraordinary claims require extraordinary evidence."

  • Sounds like the sins of the fathers will be visited on several generations...like the Bible stated 2,900 years ago.

  • Fascinating, and contributing to the increasing body of evidence around this topic. Of particular interest with reference to the impact of intergenerational trauma.

  • WHY DO THESE ARTICLES NEVER SAY WHEN THEY WERE WRITTEN! How are we supposed to believe this info, if you can't even say when you wrote it.🙄

  • Uuugh. I reloaded the link. Now it seems to arrive with all the content. Including the date written.🙂

  • I have a question. How does the gene of the sperm get influenced? If my father donates a sperm cell with certain genes while he is experiencing experiences in his life that are ABC will it create a different offspring with a different histone than one he donated while experiencing a different experience in life that is XYZ? If I was conceived during a time of wealth happiness and provision does it differ from if I was conceived during a time of grief struggle and hardship in my fathers life? Are the host ones different from sperm to sperm or does it only depend on the histone he received from his father who is my grandfather?

  • I see this SciTechDaily article is dated October 4, 2022 -- the date appears at the *bottom* if the page.

  • However epigenetics actually works, we all know it works. It's the actual shadow part of our deep shadow work. Just as past stressful periods developed generations, this current period of historic stress is already in motion. Socialpsycho researchers will have much evidence for new insights

  • The Bible has said this for centurias. As a matter of fact is mentions that genétic memory can go back as far as 4 generations!!! Imagine that.

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University of California - Santa Cruz

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