Xenobots: Scientists Build the First-Ever Living Robots That Can Reproduce

AI Designed Xenobot

An AI-designed “parent” organism (C shape; red) beside stem cells that have been compressed into a ball (“offspring”; green). Credit: Douglas Blackiston and Sam Kriegman

AI-designed Xenobots reveal entirely new form of biological self-replication—promising for regenerative medicine.

To persist, life must reproduce. Over billions of years, organisms have evolved many ways of replicating, from budding plants to sexual animals to invading viruses.

Now scientists have discovered an entirely new form of biological reproduction — and applied their discovery to create the first-ever, self-replicating living robots.

The same team that built the first living robots (“Xenobots,” assembled from frog cells — reported in 2020) has discovered that these computer-designed and hand-assembled organisms can swim out into their tiny dish, find single cells, gather hundreds of them together, and assemble “baby” Xenobots inside their Pac-Man-shaped “mouth” — that, a few days later, become new Xenobots that look and move just like themselves.

And then these new Xenobots can go out, find cells, and build copies of themselves. Again and again.

“With the right design — they will spontaneously self-replicate,” says Joshua Bongard, a computer scientist and robotics expert at the University of Vermont who co-led the new research.

The results of the new research were published November 29, 2021, in the Proceedings of the National Academy of Sciences.

Into the Unknown

In a Xenopus laevis frog, these embryonic cells would develop into skin. “They would be sitting on the outside of a tadpole, keeping out pathogens and redistributing mucus,” says Michael Levin, a professor of biology and director of the Allen Discovery Center at Tufts University and co-leader of the new research. “But we’re putting them into a novel context. We’re giving them a chance to reimagine their multicellularity.”

And what they imagine is something far different than skin. “People have thought for quite a long time that we’ve worked out all the ways that life can reproduce or replicate. But this is something that’s never been observed before,” says co-author Douglas Blackiston, the senior scientist at Tufts University who assembled the Xenobot “parents” and developed the biological portion of the new study.

“We’ve discovered that there is this previously unknown space within organisms, or living systems, and it’s a vast space.” — Josh Bongard

“This is profound,” says Levin. “These cells have the genome of a frog, but, freed from becoming tadpoles, they use their collective intelligence, a plasticity, to do something astounding.” In earlier experiments, the scientists were amazed that Xenobots could be designed to achieve simple tasks. Now they are stunned that these biological objects—a computer-designed collection of cells — will spontaneously replicate. “We have the full, unaltered frog genome,” says Levin, “but it gave no hint that these cells can work together on this new task,” of gathering and then compressing separated cells into working self-copies.

“These are frog cells replicating in a way that is very different from how frogs do it. No animal or plant known to science replicates in this way,” says Sam Kriegman, the lead author on the new study, who completed his PhD in Bongard’s lab at UVM and is now a post-doctoral researcher at Tuft’s Allen Center and Harvard University’s Wyss Institute for Biologically Inspired Engineering.

AI Designed Organisms

AI-designed (C-shaped) organisms push loose stem cells (white) into piles as they move through their environment. Credit: Douglas Blackiston and Sam Kriegman

On its own, the Xenobot parent, made of some 3,000 cells, forms a sphere. “These can make children but then the system normally dies out after that. It’s very hard, actually, to get the system to keep reproducing,” says Kriegman. But with an artificial intelligence program working on the Deep Green supercomputer cluster at UVM’s Vermont Advanced Computing Core, an evolutionary algorithm was able to test billions of body shapes in simulation — triangles, squares, pyramids, starfish — to find ones that allowed the cells to be more effective at the motion-based “kinematic” replication reported in the new research.

“We asked the supercomputer at UVM to figure out how to adjust the shape of the initial parents, and the AI came up with some strange designs after months of chugging away, including one that resembled Pac-Man,” says Kriegman. “It’s very non-intuitive. It looks very simple, but it’s not something a human engineer would come up with. Why one tiny mouth? Why not five? We sent the results to Doug and he built these Pac-Man-shaped parent Xenobots. Then those parents built children, who built grandchildren, who built great-grandchildren, who built great-great-grandchildren.” In other words, the right design greatly extended the number of generations.

Xenobot Reproducing

An AI-designed, Pac-Man-shaped “parent” organism (in red) beside stem cells that have been compressed into a ball—the “offspring” (green). Credit: Douglas Blackiston and Sam Kriegman

Kinematic replication is well-known at the level of molecules — but it has never been observed before at the scale of whole cells or organisms.

“We’ve discovered that there is this previously unknown space within organisms, or living systems, and it’s a vast space,” says Bongard, a professor in UVM’s College of Engineering and Mathematical Sciences. “How do we then go about exploring that space? We found Xenobots that walk. We found Xenobots that swim. And now, in this study, we’ve found Xenobots that kinematically replicate. What else is out there?”

Or, as the scientists write in the Proceedings of the National Academy of Sciences study: “life harbors surprising behaviors just below the surface, waiting to be uncovered.”

Xenobot Parents

As Pac-man-shaped Xenobot “parents” move around their environment, they collect loose stem cells in their “mouths” that, over time, aggregate to create “offspring” Xenobots that develop to look just like their creators. Credit: Doug Blackiston and Sam Kriegman

Responding to Risk

Some people may find this exhilarating. Others may react with concern, or even terror, to the notion of a self-replicating biotechnology. For the team of scientists, the goal is deeper understanding.

“We are working to understand this property: replication. The world and technologies are rapidly changing. It’s important, for society as a whole, that we study and understand how this works,” says Bongard. These millimeter-sized living machines, entirely contained in a laboratory, easily extinguished, and vetted by federal, state and institutional ethics experts, “are not what keep me awake at night. What presents risk is the next pandemic; accelerating ecosystem damage from pollution; intensifying threats from climate change,” says UVM’s Bongard. “This is an ideal system in which to study self-replicating systems. We have a moral imperative to understand the conditions under which we can control it, direct it, douse it, exaggerate it.”

Xenobot Research Team

Xenobots are an invention of and collaborative research effort by (from left): Josh Bongard, University of Vermont; Michael Levin, Tufts University and the Wyss Institute at Harvard University; Douglas Blackiston, Tufts University; and Sam Kriegman, Tufts University and the Wyss Institute at Harvard University. Credit: Tufts and ICDO

Bongard points to the COVID epidemic and the hunt for a vaccine. “The speed at which we can produce solutions matters deeply. If we can develop technologies, learning from Xenobots, where we can quickly tell the AI,: ‘We need a biological tool that does X and Y and suppresses Z,’ —that could be very beneficial. Today, that takes an exceedingly long time.” The team aims to accelerate how quickly people can go from identifying a problem to generating solutions—”like deploying living machines to pull microplastics out of waterways or build new medicines,” Bongard says.

“We need to create technological solutions that grow at the same rate as the challenges we face,” Bongard says.

And the team sees promise in the research for advancements toward regenerative medicine. “If we knew how to tell collections of cells to do what we wanted them to do, ultimately, that’s regenerative medicine—that’s the solution to traumatic injury, birth defects, cancer, and aging,” says Levin. “All of these different problems are here because we don’t know how to predict and control what groups of cells are going to build. Xenobots are a new platform for teaching us.”

Reference: “Kinematic self-replication in reconfigurable organisms” by Sam Kriegman, Douglas Blackiston, Michael Levin, and Josh Bongard, 29 November 2021, .
DOI: 10.1073/pnas.2112672118

18 Comments on "Xenobots: Scientists Build the First-Ever Living Robots That Can Reproduce"

  1. this is scary.

  2. “We have a moral imperative to understand the conditions under which we can control it”… How bad is it going to hurt the first time they figure out the conditions under which they can’t control it?

  3. Lrr of the planet Omicron Persei 8 | November 30, 2021 at 6:17 am | Reply

    Reminds me of popplers

  4. Isn’t this the plotline to Agent Cody Banks? I remember that nanorobots that replicate themselves by tearing down cells and building new nanorobots was a plot in one of the Cody Banks movies

  5. Sounds like computers meet Corona Virus to me, I don’t think Computers have made the world better nor Corona Virus . People die immortality is the pursuit of the dim-witted. People need to live and die, to work to strive to think for themselves.This is more folly from people who are too arrogant to concede their ignorance. I do admit I am ignorant of so many things but I am not too ignorant to see why this is not going to end well.

  6. Concerned Human | November 30, 2021 at 10:14 am | Reply

    STOP PLAYING WITH NATURE!

  7. David Peterson | November 30, 2021 at 3:00 pm | Reply

    Robots are machines. These are not. Another falsely titled article.

  8. Just a Comment | November 30, 2021 at 7:47 pm | Reply

    Scientists always think they can control something and invariably, they are always proven wrong. It is odd how many scientists who do not believe in God so often want to play God.

    Hate to tell you, there is only one God and we shouldn’t be monkeying around with his creation. By the way, in Aliens the monster was called a Xenomorph. Now, we have Xenobots which are cells apparently. In the movies, they too tried to control the monster and well, it never ends well for mankind.

  9. You do not have a moral obligation to study and understand it. You have a moral obligation to stop. You have an ego driven greed to continue for wealth and fame. May you get everything you deserve.

  10. Ah look at that last picture, it’s the four horsemen of the apocalypse …

  11. What’s with all these religious nuts? Is this site like a congregation spot?

    • I don’t think so. Comments posted are from individual personal perspectives, just like your questions. Individuals are free to accept, attack or dismiss as their perspective dictates. A bit of irony in your chosen nom de plume in light of your questions as one could choose to make a connection to another rather famous Magdalene in a manuscript one may freely choose to accept as fact or fiction.

  12. What an incredible collection of examples of people with absolutely no understanding of a topic making bold statements that are not grounded in reality. These cells cannot spontaneously develop dangerous abilities, you nut jobs watch a few Sci fi movies and think you know something about science, forgetting what the fi stands for, lmao.

  13. no way

  14. help m…e hill

  15. … well, if those robots can carry molecules of the gold to the cancer cells then one could use microwaves to eliminate the cancer…

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