Powerhouse Puzzle Solved: How a Massive Supercomplex in Mitochondria Shapes Cellular Respiration

The Complete Respiratory Supercomplex

A research article in Nature has shed light on the structure and function of respiratory supercomplexes in cellular respiration, particularly in a single-cell eukaryote, Tetrahymena thermophila. The study combined various techniques to demonstrate that all four respiratory complexes in this organism associate together to form a massive supercomplex consisting of 150 proteins and 311 lipids, which significantly influences the shape of the mitochondrial membrane. Credit: luminous-lab.com

A new study has revealed how respiratory supercomplexes, consisting of four associated respiratory complexes, shape the mitochondrial membrane in the single-cell eukaryote, Tetrahymena thermophila. The research showed these supercomplexes have both an enzymatic and structural function, optimizing ATP synthesis and supporting energy conversion. The discovery of a split and extended subunit, COX3, highlights an evolutionary mechanism beneficial for inter-complex contacts.

Eukaryotes generate the energy for survival through cellular respiration in mitochondria by a process known as the oxidative phosphorylation. In this process, nutrients and oxygen are converted into a chemical form of energy – the ATP. This is achieved with a proton gradient built up by the electron transport chain inside mitochondria. The gradient is driven by a series of four respiratory complexes in the inner mitochondrial membrane.

A study published in the journal Nature combined single-particle, tomography, molecular simulations and biophysics to shed light on bioenergetic macro-assemblies and how they shape mitochondrial membranes. It identified that In Tetrahymena thermophila — a free-living single cell eukaryote found in ponds and lakes, all four respiratory complexes are associated together. They form a massive 5.8 megadalton supercomplex of 150 proteins with at least 300 transmembrane helices and 311 lipids. Owing to subunit acquisition and extension, Complex I binds a dimer of Complex III that is tilted by 37 degrees. Complex I also associates with Complex IV dimer, generating a gap that serves as a binding site for Complex II. The study demonstrates that this assembly is crucial to the shaping of the bioenergetic membrane.


The evolution of protein subunits of respiratory complexes has led to the I–II–III2–IV2 assembly that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization.

One of the most intriguing findings is that a subunit of Complex IV called COX3 is split in two. The fragmentation occurs on the genetic level, and then each fragment is extended contributing to some of the interfaces between complexes. The gain of function for inter-complex contacts represents an evolutionary mechanism, showing how neutral molecular complexity can become beneficial.

The findings highlight how the evolution of protein subunits of respiratory complexes has led to the suercomplex assembly, which actively contributes to mitochondrial membrane curvature induction, which is necessary for proper mitochondrial function. This way, the supercomplex shapes the macroscopic architecture of mitochondria, ultimately optimizing ATP synthesis. Therefore, respiratory supercomplexes have not only an enzymatic but also a structural function of shaping the membrane, and both together support energy conversion and provide fuel for life.

For more on this research, see Scientists Identify Complete Respiratory Supercomplex.

Reference: “Structural basis of mitochondrial membrane bending by the I–II–III2–IV2 supercomplex” by Alexander Mühleip, Rasmus Kock Flygaard, Rozbeh Baradaran, Outi Haapanen, Thomas Gruhl, Victor Tobiasson, Amandine Maréchal, Vivek Sharma and Alexey Amunts, 22 March 2023, Nature.
DOI: 10.1038/s41586-023-05817-y

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