Biologists Reveal Key Mechanism Behind the Formation of Spider Silk

Key Mechanism Behind the Formation of Spider Silk

A Nephila clavata producing silk.

A group of scientists led by researchers from the RIKEN Center for Sustainable Resource Science (CSRS) have examined the soluble precursor of spider silk and found that a previously undiscovered structural element is key to how the proteins form into the beta-sheet conformation that gives the silk its exceptional strength.

Spider silk is known for its exceptional toughness and flexibility. It is several times stronger than steel, and yet is much more flexible. As a result, efforts are being made by scientists around the world to try to develop analogs that could be used in industrial and medical applications. However, though it is known that the beta-sheets in spider silk are key to its strength, how the sheets are formed is poorly understood, making it difficult to create artificial variants. Part of the reason it is difficult to understand the mechanism is that the silk is initially created as soluble proteins, which very quickly crystallize into a solid form, and it has been very difficult to analyze the soluble form.

To elucidate this, the CSRS researchers generated silk proteins using genetically modified bacteria that can produce silk from a golden orb-web spider (Nephila clavipes), and then performed complex analyses of the soluble proteins. They looked particularly at the repeating elements that are enclosed between two terminal elements that have been well characterized. They found that the repeating domain is composed of two patterns–random coils and a pattern called polyproline type II helix. It turns out that the second type is crucial for the formation of strong silk.

Essentially, their studies demonstrated that the polyproline type II helix can form a rigid structure which can then be transformed into beta-sheets very quickly, allowing the silk to be quickly woven. Intriguingly, it turned out that pH–which is thought to be important for the molecular interactions of the N- and C-terminal domains–does not play an important part of the folding of the repetitive domains, and that it is rather the removal of water and mechanical forces as the precursor moves through the silk gland.

According to Nur Alia Oktaviani, the first author of the study, “We were fortunate to be able to use a combination of powerful methods, including solution-state nuclear magnetic resonance spectroscopy, far-UV circular dichroism spectroscopy, and vibrational circular dichroism spectroscopy, to analyze the protein before it formed into the beta-sheets. It was very satisfying to discover this special conformation that leads to the formation of the beta-sheets.”

According to Keiji Numata, who is a project leader of JST ImPACT and led the research group, “Spider silk is a wonderful material, as it is extremely tough but does not contain harmful substances and is readily biodegradable, so it does not exert any harmful load on the environment. We hope that this discovery will help make it possible to create artificial silk that will prove useful for society.”

Reference: “Conformation and dynamics of soluble repetitive domain elucidates the initial β-sheet formation of spider silk” by Nur Alia Oktaviani, Akimasa Matsugami, Ali D. Malay, Fumiaki Hayashi, David L. Kaplan and Keiji Numata, 29 May 2018, Nature Communications.
DOI: 10.1038/s41467-018-04570-5

1 Comment on "Biologists Reveal Key Mechanism Behind the Formation of Spider Silk"

  1. Spider Silk is amazing, but for some reason i fear the 8 legged crawlers, Without permission, As a kid building an awsome tree house in the back yard using my dads good timber & nails wood saw & hammer, from an over protruding nail my hand somehow got caught up on it so i ripped my hand away from it, upon which i recieved an awful open gashed wound, bleeding I rushed to my dad “awwh its only a scratch son” but what my dad proceeded to do was, he went about amongst the hedges & tree branches gathered up a large amount of spider silk {spider web} applied it onto my injury then bandaged it, then told me tidy up my mess. bleeding stopped almost instantly, I was kind of somewhat discussted & amazed & very intrigued, my dad has since taught & instructed me on a few amazing medical use’s of spider silk, prepare & apply,

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