Health

Powerful New Antibody Neutralizes All Known COVID Variants

The antibody could greatly improve our ability to defend against future variants.

Future vaccine development may be inspired by the findings.

Therapeutic antibodies that were effective early in the pandemic have lost their efficacy as SARS-CoV-2 has changed and mutated, and more recent variants, particularly Omicron, have learned how to circumvent the antibodies our systems produce in response to vaccinations. We may be able to better guard against possible variations thanks to a new, widely neutralizing antibody created at Boston Children’s Hospital. In tests, it neutralized all known SARS-CoV-2 variants of concern, including all Omicron variants.

“We hope that this humanized antibody will prove to be as effective at neutralizing SARS-CoV-2 in patients as it has proven to be thus far in preclinical evaluations,” says Frederick Alt, Ph.D., of the Program in Cellular and Molecular Medicine at Boston Children’s Hospital, who co-led the research.

In a study that was recently published in Science Immunology, Alt and Sai Luo, Ph.D., utilized a modified version of a humanized mouse model that his lab had previously used to look for broadly neutralizing antibodies to HIV, another virus that often mutates. Since the mice effectively have built-in human immune systems, the model closely resembles how the trial-and-error process our immune system uses to create increasingly effective antibodies.

The researchers initially introduced two human gene segments into the mice, causing their B cells to create a wide repertoire of humanized antibodies in a short period of time. They subsequently exposed the mice to the original Wuhan-Hu-1 strain of the virus’s SARS-CoV-2 spike protein, which is the main protein targeted by our antibodies and current vaccines. The modified mice developed nine lineages, or “families,” of humanized antibodies that bonded to the spike in response.

Together with a Duke University team led by Dr. Barton Haynes, Alt and Luo then assessed the efficacy of these antibodies. Antibodies from three of the nine lineages were effective in neutralizing the original Wuhan-Hu-1 virus. The SP1-77 antibody and other members of its lineage, in particular, demonstrated extremely wide activity, neutralizing Alpha, Beta, Gamma, Delta, and all prior and current Omicron strains.

A new approach to virus neutralization

What caused the SP1-77 antibody to be so broadly neutralizing? Structural studies by a collaborating team led by Bing Chen, Ph.D. and Jun Zhang, Ph.D. at Boston Children’s Hospital and the Haynes group at Duke, showed that SP1-77 works differently from current antibodies (either therapeutic antibodies or those we make in response to current vaccines).

Many of the existing antibodies work by attaching to the receptor-binding domain (RBD) of the spike in certain regions, preventing SARS-CoV-2 from binding to our cells’ ACE2 receptors, which is the initial step in infection. The SP1-77 antibody binds to the RBD as well, but in a completely different manner that does not prevent the virus from binding to ACE2 receptors.

Using a novel live-cell imaging platform described in a preprint, collaborators Alex Kreutzberger, Ph.D. and Tomas Kirchhausen, Ph.D., of Boston Children’s Hospital showed that SP1-77 prevents the virus from fusing its outer membrane with the membrane of the target cell. This thwarts the final necessary step that throws the door open to infection.

These features may inform the design of new SARS-CoV-2 vaccines. “SP1-77 binds the spike protein at a site that so far has not been mutated in any SARS-CoV-2 variant, broadly neutralizing current variants by a novel mechanism,” says Kirchhausen.

Reference: “An Antibody from Single Human VH-rearranging Mouse Neutralizes All SARS-CoV-2 Variants Through BA.5 by Inhibiting Membrane Fusion” by Sai Luo, Jun Zhang, Alex J.B. Kreutzberger, Amanda Eaton, Robert J. Edwards, Changbin Jing, Hai-Qiang Dai, Gregory D. Sempowski, Kenneth Cronin, Robert Parks, Adam Yongxin Ye, Katayoun Mansouri, Maggie Barr, Novalia Pishesha, Aimee Chapdelaine Williams, Lucas Vieira Francisco, Anand Saminathan, Hanqin Peng, Himanshu Batra, Lorenza Bellusci, Surender Khurana, S. Munir Alam, David C. Montefiori, Kevin O. Saunders, Ming Tian, Hidde Ploegh, Tom Kirchhausen, Bing Chen, Barton F. Haynes and Frederick W. Alt, 11 August 2022, Science Immunology.
DOI: 10.1126/sciimmunol.add5446

The study was funded by the Howard Hughes Medical Institute, the Bill & Melinda Gates Foundation, the NIH NIAID Consortia for HIV/AIDS Vaccine Development, the Massachusetts Consortium on Pathogen Readiness, Emergent Ventures, the Food and Drug Administration, the NIH Maximizing Investigators’ Research Award, NIH Grant AI163019, the Danish Technical University and SANA, IONIS, and a Harvard Virology Program NIH training grant.

Alt and Ming Tian, Ph.D., at Boston Children’s are authors of a patent application describing the mouse model. Luo, Haynes, and Alt are authors of patent applications describing the antibodies.

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  • Searching for an antibody that will neutralize all pathogens is like Searching for a key that will open all locks. Antigen/antibody binding is a lock and key phenomenon. An antibody production is provoked by specific Antigenic stimulation. Hence, each antibody will bind and neutralize the specific Antigen that provoke its production. This is fundamental immunological principle. To go in Search of an all conquering vaccine that will provoke the production of an all conquering antibody, is intellectually dishonest.

  • Felix Moyo Edonmi... thats not true and when you say it is intellectually dishonest, exactly what are your qualifications for yourself being any kind of expert or at minimum even an intellectual about the subject? Just off the top of my head, I can name that shark antibodies don't operate by binding and neutralization as you state. Shark antibodies will tear thru a cell structure. These are humanized mice antibodies and you are just assuming for some reason that since most purely human antibodies just a binding/neutralization mechanism that all antibodies must. That is a failure of both logic and knowledge on your part.

  • If we stop the pathogen from fusing its outer membrane with the membrane of our cells thus blocking the lock for all keys what method would a virus use to circumvent that which would be all conquering?

  • Lest we forget that the protein that has not YET mutated might in the future. So the best outcome is we can through SP1-77 systhesis force the "species " of virus to extinction. (no hosts no spread no spread extinction. This assumes SP1-77 antibody is given to everyone including the new born ??

    • A combination of this vaccine and strict infection-control methods could hypothetically eliminate Sars-CoV-2.

      One consequence of strict measures to control the spread of Sars-CoV-2 in 2020 was the coincident control of the spread of other airborne viruses. It appears to have eliminated one of the two known lineages of Influenza B. B/Yamagata has not been found in any sample collected since the winter of 2020-21.

      In practice, given the politicization and conspiracy theories of vaccination and infection control measures for Sars-CoV-2, there is no hope of us eliminating it.

  • “SP1-77 binds the spike protein at a site that so far has not been mutated in any SARS-CoV-2 variant, broadly neutralizing current variants by a novel mechanism,” says Kirchhausen. The virus has not been subjected to these antibodies to any broad degree.
    We can only hope that the membrane fusion mechanism depends on the proteins involved in the fusion to be so invariant that any mutation will doom the virus's infectivity. Otherwise, it may be possible that the selective pressure of exposure to these antibodies may result in new, viable mutations to arise.

  • This would be a modern development of great importance but for the time being, "ignorance and anti-vaxers" is the biggest problem that we have when it comes to mitigating COVID or any other virus.

  • This doesn't explain if this vaccine would prevent further mutations. And if it doesn't, would there be a risk of a more severe strain of the virus. I'm also confused at how fast COVID has mutated.With HIV, if I recall it took some time to mutate. Also my concern is, how do we know that the current vaccines are not responsible or partly responsible for these rapid mutations? This article is more redundant than informative.

    • All RNA based viruses mutate quickly, including Sars-CoV-2, Influenza, and HIV. Sars-CoV-2 is not wildly out of line with other RNA viruses.

      In the case of HIV, reverse transcriptase is very error prone, and thus prone to introducing new mutations.

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Boston Children's Hospital

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