Trigger for the Onset of Alzheimer’s Disease Identified

Researchers Pinpoint a Catalytic Trigger for the Onset of Alzheimers

Image, magnified a million times, of amyloid fibril, the type of protein structures that are formed in Alzheimer’s. Credit: Dr. Tuomas Knowles

Scientists have pinpointed a catalytic trigger for the onset of Alzheimer’s disease, establishing the pathway that shows how the toxic species that cause cell death, the oligomers, are formed.

Researchers have pinpointed a catalytic trigger for the onset of Alzheimer’s disease – when the fundamental structure of a protein molecule changes to cause a chain reaction that leads to the death of neurons in the brain.

For the first time, scientists at Cambridge’s Department of Chemistry have been able to map in detail the pathway that generates “aberrant” forms of proteins that are at the root of neurodegenerative conditions such as Alzheimer’s.

They believe the breakthrough is a vital step closer to increased capabilities for earlier diagnosis of neurological disorders such as Alzheimer’s and Parkinson’s, and opens up possibilities for a new generation of targeted drugs, as scientists say they have uncovered the earliest stages of the development of Alzheimer’s that drugs could possibly target.

The study, published today in the journal PNAS, is a milestone in the long-term research established in Cambridge by Professor Christopher Dobson and his colleagues, following the realization by Dobson of the underlying nature of protein ‘misfolding’ and its connection with disease over 15 years ago.

The research is likely to have a central role to play in diagnostic and drug development for dementia-related diseases, which are increasingly prevalent and damaging as populations live longer.

“There are no disease-modifying therapies for Alzheimer’s and dementia at the moment, only limited treatment for symptoms. We have to solve what happens at the molecular level before we can progress and have real impact,” said Dr. Tuomas Knowles, lead author of the study and long-time collaborator of Professor Dobson.

“We’ve now established the pathway that shows how the toxic species that cause cell death, the oligomers, are formed. This is the key pathway to detect, target, and intervene – the molecular catalyst that underlies the pathology.”

In 2010, the Alzheimer’s Research Trust showed that dementia costs the UK economy over £23 billion, more than cancer and heart disease combined. Just last week, PM David Cameron urged scientists and clinicians to work together to “improve treatments and find scientific breakthroughs” to address “one of the biggest social and healthcare challenges we face.”

The neurodegenerative process giving rise to diseases such as Alzheimer’s is triggered when the normal structures of protein molecules within cells become corrupted.

Protein molecules are made in cellular ‘assembly lines’ that join together chemical building blocks called amino acids in an order encoded in our DNA. New proteins emerge as long, thin chains that normally need to be folded into compact and intricate structures to carry out their biological function.

Under some conditions, however, proteins can ‘misfold’ and snag surrounding normal proteins, which then tangle and stick together in clumps which build to masses, frequently millions, of malfunctioning molecules that shape themselves into unwieldy protein tendrils.

The abnormal tendril structures, called ‘amyloid fibrils’, grow outwards around the location where the focal point, or ‘nucleation’ of these abnormal “species” occurs.

Amyloid fibrils can form the foundations of huge protein deposits – or plaques – long-seen in the brains of Alzheimer’s sufferers, and once believed to be the cause of the disease, before the discovery of ‘toxic oligomers’ by Dobson and others a decade or so ago.

A plaque’s size and density renders it insoluble, and consequently unable to move. Whereas the oligomers, which give rise to Alzheimer’s disease, are small enough to spread easily around the brain – killing neurons and interacting harmfully with other molecules – but how they were formed was until now a mystery.

The new work, in large part carried out by researcher Samuel Cohen, shows that once a small but critical level of malfunctioning protein ‘clumps’ have formed, a runaway chain reaction is triggered that multiplies exponentially the number of these protein composites, activating new focal points through ‘nucleation’.

It is this secondary nucleation process that forges juvenile tendrils, initially consisting of clusters that contain just a few protein molecules. Small and highly diffusible, these are the ‘toxic oligomers’ that careen dangerously around the brain cells, killing neurons and ultimately causing loss of memory and other symptoms of dementia.

The researchers brought together kinetic experiments with a theoretical framework based on master equations, tools commonly used in other areas of chemistry and physics but had not been exploited to their full potential in the study of protein malfunction before.

The latest research follows hard on the heels of another groundbreaking study, published in April of this year again in PNAS, in which the Cambridge group, in Collaboration with Colleagues in London and at MIT, worked out the first atomic structure of one of the damaging amyloid fibril protein tendrils. They say the years spent developing research techniques are really paying off now, and they are starting to solve “some of the key mysteries” of these neurodegenerative diseases.

“We are essentially using physical and chemical methods to address a biomolecular problem, mapping out the networks of processes and dominant mechanisms to ‘recreate the crime scene’ at the molecular root of Alzheimer’s disease,” explained Knowles.

“Increasingly, using quantitative experimental tools and rigorous theoretical analysis to understand complex biological processes are leading to exciting and game-changing results. With a disease like Alzheimer’s, you have to intervene in a highly specific manner to prevent the formation of the toxic agents. Now we’ve found how the oligomers are created, we know what process we need to turn off.”

Reference: “Proliferation of amyloid-β42 aggregates occurs through a secondary nucleation mechanism” by Samuel I. A. Cohen, Sara Linse, Leila M. Luheshi, Erik Hellstrand, Duncan A. White, Luke Rajah, Daniel E. Otzen, Michele Vendruscolo, Christopher M. Dobson and Tuomas P. J. Knowles, 23 May 2013, Proceedings of the National Academy of Sciences.
DOI: 10.1073/pnas.1218402110

2 Comments on "Trigger for the Onset of Alzheimer’s Disease Identified"

  1. Madanagopal.V.C | May 22, 2013 at 1:13 am | Reply

    Ameloid-B plaques which was supposed to cause Alzheimer’s disease is due to wrong functioning of intra-cellular ‘chaperones’ the folding messenger for protein fibrils which comes as a long thread. ‘Chaperones’ misbehave only with aging process just as cellular ‘lysosomes’ fail to clear the cellular debris in aging cells. If the toxic’ oligomers’ which are formed as intra-cellular bodies by the secondary nucleation of juvinelle fibrils of micro proteins is also found to be a cause of Alzheimer’s is it not the usual behavior of cytoplasmic materials undergoing aging process only? Thank You.

  2. Those who’ve come to understand the potential value of unasked-for input from SOME (few?) of those with backgrounds COMPLETELY alien to one’s accomplished, intense, intellectual focus(read: -book- Creative Intelligence), MIGHT find themselves averse to dismissing my post here, with an arrogant “sniff”.

    RE; “…Once a small but critical level of malfunctioning protein ‘clumps’ have formed, a runaway chain reaction is triggered that multiplies exponentially the number of these protein composites, activating new focal points through ‘nucleation’…”

    LESS pronounced Epigenetic factors notwithstanding, NO thoughtful, educated, intelligent individual or clinical practitioner should fail to read and think-through the implications of those athletes or victims of head-trauma presenting with the Apolipoprotein-4 (APO-E4) gene…

    Subdural hematoma…could THAT provide insta-malfunctioning protein ‘clumps’?!

    CLEARLY- The mass of people developing this horrible disease haven’t necessarily been deliberately or accidentally knocked unconscious, however- what % of them who have, went on to develop Alzheimer’s Disease?!

    With modern genetic testing availability, who should NOT be allowed to participate in “contact sports”?!

    I am 56, was a successful Boxer and Wrestler. I was in MANY violent street fights as a kid, and also played football. I suspect that the APO-E4 gene isn’t in my profile. However- to how many and who is it?!

    Of PARTICULAR disgust to me, is the evolution of “Cage Fighting”. MMA.

    Run with this like a research bitch and yield no attribution to it as thought-genesis, if you’re mired in no-ethics / morality, “modern moral relativism”. Alternatively, please keep me in the loop and trust to goodness, “karma”, “what goes around, comes around”, wisdom* etc.

    Best regards to the deserved,



    William Shakespeare Act 1, Scene 5

    “…And therefore as a stranger give it welcome.
    There are more things in heaven and earth, Horatio,
    Than are dreamt of in your philosophy….

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