International Team of Scientists Reveal a New Type of Botox

A new source of the botulinum neurotoxin was discovered by Canadian and American scientists in a strain of animal gut bacteria known as Enterococcus faecium. The neurotoxic protein is known for its paradoxical ability to remove wrinkles yet cause botulism, a potentially fatal illness associated with food poisoning.

Over the past 20 years, there has also been a growing number of therapeutic applications for botulinum toxin type A, known as Botox, including treatment for migraines, leaky bladders, excessive sweating, and cardiac conditions.

“This is the first time that an active botulinum toxin has been identified outside of Clostridium botulinum and its relatives, which are often found in soil and untreated water,” said Andrew Doxey, one of the study’s two corresponding authors and a bioinformatics professor at the University of Waterloo. “Its discovery has implications in several fields, from monitoring the emergence of new pathogens to the development of new protein therapeutics–it’s a game changer.”

Doxey’s findings were developed in collaboration with researchers from Harvard University and the Boston Children’s Hospital.

In the study, originally designed to investigate the origins of antibiotic resistance in E. faecium bacteria, the researchers were able to sequence the genome of the E. faecium bacteria drawn from cow feces. The genome was then run through computer programs in Doxey’s lab, which found the gene for botulinum toxin in the bacterial strain.

The researchers concluded that the botulinum toxin was likely transferred from C. botulinum bacteria in the environment into the E. faecium bacteria in the cow’s gut, showing that the toxin can be transferred between very different species.

“The botulinum toxin is a powerful and versatile protein therapeutic,” says Michael Mansfield, a Biology doctoral candidate in the Doxey Lab and one of the study’s lead authors. “By finding more versions of the toxin in nature, we can potentially expand and optimize its therapeutic applications even further.”

Reference: “Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium” by Sicai Zhang, Francois Lebreton, Michael J. Mansfield, Shin-Ichiro Miyashita, Jie Zhang, Julia A. Schwartzman, Liang Tao, Geoffrey Masuyer, Markel Martínez-Carranza, Pål Stenmark, Michael S. Gilmore, Andrew C. Doxey and Min Dong, 30 January 2018, Cell Host & Microbe.
DOI: 10.1016/j.chom.2017.12.018
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