University of Chicago scientists have expanded our understanding of snoRNAs, discovering their extensive influence on cellular functions beyond guiding RNA modifications.
Their work introduces potential therapeutic applications for controlling protein secretion, highlighting snoRNAs’ broader biological significance.
snoRNAs and Their Functions
Dynamic and reversible modifications to DNA and RNA play a critical role in controlling gene expression and transcription, influencing cellular processes, disease progression, and overall health. Small nucleolar RNAs (snoRNAs), a commonly overlooked group of guide RNA molecules, direct chemical modifications to ribosomal RNA (rRNA), much like an usher guiding someone to their seat.
Researchers at the University of Chicago have developed an innovative method to identify new RNA targets for snoRNAs. Using this approach, they uncovered thousands of previously unknown snoRNA targets in human cells and mouse brain tissues. Remarkably, many of these targets have functions beyond rRNA modification. Among the discoveries are snoRNA interactions with messenger RNA (mRNA) that aid in protein secretion—an essential cellular process with significant potential for therapeutic and biotechnological applications.
snoRNA’s Role in Protein Secretion
“Once you see so many targets for these snoRNAs, you realize there’s a lot more to be understood,” said Chuan He, PhD, John T. Wilson Distinguished Service Professor of Chemistry and Professor of Biochemistry and Molecular Biology at the University of Chicago and co-senior author of the paper. “We already see that they play a role in protein secretion, which has major implications for physiology, and it suggests a path forward to study hundreds of other snoRNAs.”
The paper, “SnoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification,” was published in November 2024 in the journal Cell.
Advances in snoRNA Research and Applications
There are more than 1,000 known genes for encoding snoRNAs in the human genome, but scientists have only pinpointed the RNA targets for about 300 of them. These targets mostly involve guiding modifications for ribosomal RNA and small nuclear RNA involved in mRNA splicing. In the decades since snoRNAs were first discovered, researchers largely left the remaining 700 alone, assuming they performed similar functions. However, unlike other guide RNA molecules such as microRNAs that are all the same length, snoRNAs vary greatly in their length from 50-250 residues, suggesting that they can do many different things.
Over the past 12 years, He’s lab has developed several biochemical and sequencing techniques for studying transcription, DNA modifications, and RNA modifications. In the new study, He worked with co-senior author Tao Pan, PhD, Professor of Biochemistry and Molecular Biology, to test a new tool called “snoKARR-seq” that links snoRNAs with their target binding RNAs. Bei Liu, PhD, a Chicago Fellow postdoctoral scholar who is co-mentored by He and Pan, led the project.
“Chuan’s lab developed this killer technology to look at exactly what RNA each snoRNA is interacting with at the transcriptome level,” Pan said. “Now there’s a lot of open space for understanding comprehensively what these 1,000 human genes [that encode snoRNAs] are doing.”
The Unexpected Role of SNORA73 in Cellular Processes
Most of the newly discovered snoRNA targets do not overlap with the known RNA modification sites, suggesting that snoRNAs may have a much broader function in cells. One unexpected discovery was that a snoRNA called SNORA73 interacts with mRNAs that encode secreted proteins and cell membrane proteins. Protein secretion is a fundamental biological process by which proteins are transported from a cell into the extracellular space, which is crucial for various functions, including communication between cells, immune responses, and digestion. The researchers saw that SNORA73 acts as a “molecular glue” between the mRNA and the protein synthesis machinery that helps facilitate this process.
Further analysis of how SNORA73 binds with mRNA suggested that synthetic snoRNA sequences can be engineered to affect protein secretion. The researchers tested this hypothesis by tweaking a green fluorescent protein (GFP) reporter to interact with SNORA73. GFPs are often introduced in cells to make them glow under certain conditions so scientists can see the effects of experiments. When the researchers expressed SNORA73 genes with the engineered GFP that can be secreted from cells, it increased protein secretion by 30 to 50% over controls.
snoRNA-Based Therapeutic Opportunities
These experiments showed that they could make use of the snoRNA machinery to manipulate the secretion of a given protein, which could be useful for developing therapeutics. For example, if a human disease involves a deficiency of secreted proteins, then bioengineers could hijack the system to deliver artificial snoRNAs to increase secretion of that protein.
Future Directions in snoRNA Research
While the technology for synthesizing and delivering snoRNAs to the right locations isn’t quite ready yet, both He and Pan feel confident those challenges can be solved since it builds upon previous advances in technology using other forms of RNA. They also believe that since snoRNAs are specific to cell types, they could have much more diverse functions—and therapeutic possibilities—elsewhere.
“Think about neuronal cells, stem cells, or cancer cells. There are just so many cell types one can study. So, I think the field is wide open,” He said. “Tao and I have been working together for more than 15 years, and it’s a great showcase of collaboration between the Biological Sciences Division and Physical Sciences Division at UChicago. This paper is another example that this kind of collaboration leads to opening a new field of biology.”
Reference: “snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification” by Bei Liu, Tong Wu, Bernadette A. Miao, Fei Ji, Shun Liu, Pingluan Wang, Yutao Zhao, Yuhao Zhong, Arunkumar Sundaram, Tie-Bo Zeng, Marta Majcherska-Agrawal, Robert J. Keenan, Tao Pan and Chuan He, 22 November 2024, Cell.
DOI: 10.1016/j.cell.2024.10.046
Additional authors on the study include Tong Wu, Bernadette A. Miao, Fei Ji, Shun Liu, Pingluan Wang, Yutao Zhao, Yuhao Zhong, Arunkumar Sundaram, Tie-Bo Zeng, Marta Majcherska-Agrawal, and Robert J. Keenan from UChicago.
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