A new bioreactor system uses hollow fibers to deliver nutrients to lab-grown tissue, enabling scalable cultured chicken meat production with realistic texture.
A new bioreactor that replicates the function of a circulatory system has enabled the production of more than 10 grams of chicken muscle tissue, advancing the development of cultured meat. By delivering oxygen and nutrients directly to the artificial tissue, the system supports sustained cell growth. The findings were published in the journal Trends in Biotechnology.
“Our study presents a scalable, top-down strategy for producing whole-cut cultured meat using a perfusable hollow fiber bioreactor,” says senior author Shoji Takeuchi of The University of Tokyo. “This system enables cell distribution, alignment, contractility, and improved food-related properties. It offers a practical alternative to vascular-based methods and may impact not only food production but also regenerative medicine, drug testing, and biohybrid robotics.”
One of the major challenges in engineering large-scale tissues is the inability of diffusion alone to deliver sufficient nutrients and oxygen across long distances. Without an integrated circulatory system, tissue thickness is typically limited to less than 1 millimeter. This limitation has made it difficult to grow larger, densely cellularized tissues, such as those needed for cultured meat or medical applications.
“We’re using semipermeable hollow fibers, which mimic blood vessels in their ability to deliver nutrients to the tissues,” Takeuchi says. “These fibers are already commonly used in household water filters and dialysis machines for patients with kidney disease. It’s exciting to discover that these tiny fibers can also effectively help create artificial tissues and, possibly, whole organs in the future.”
Hollow Fiber Bioreactor Demonstrates Breakthrough
The authors demonstrated the biofabrication of centimeter-scale chicken skeletal muscle tissues using a Hollow Fiber Bioreactor (HFB) consisting of an array of 50 hollow fibers. In addition, they implemented a robot-assisted assembly system for the fabrication of a 1,125-fiber HFB and produced whole-cut chicken meat weighing more than 10 g using chicken fibroblast cells, which make up connective tissue.
“Cultured meat offers a sustainable, ethical alternative to conventional meat,” Takeuchi says. “However, replicating the texture and taste of whole-cut meat remains difficult. Our technology enables the production of structured meat with improved texture and flavor, potentially accelerating its commercial viability. Beyond food, this platform may also impact regenerative medicine and soft robotics.”
According to Takeuchi, additional challenges for future research include determining the long-term effects of perfusion on tissue quality, adapting the technology for organ fabrication and biohybrid robotics, and further improving the mechanical properties and structural integrity of the tissue to better mimic the characteristics of natural muscle tissue.
“We overcame the challenge of achieving perfusion across thick tissues by arranging hollow fibers with microscale precision,” Takeuchi says. “Remaining challenges include improving oxygen delivery in larger tissues, automating fiber removal, and transitioning to food-safe materials. Solutions may include use of artificial oxygen carriers to mimic red blood cells, bundle-removal mechanisms that efficiently remove fibers in a single operation, and edible or recyclable hollow fibers.”
Reference: “Scalable tissue biofabrication via perfusable hollow fiber arrays for cultured meat applications” by Minghao Nie, Ai Shima, Mikihisa Yamamoto and Shoji Takeuchi, 16 April 2025, Trends in Biotechnology.
DOI: 10.1016/j.tibtech.2025.02.022
The authors have patent applications (PCT/JP2022/047671 and WO2023120710A1) related to this work.
Never miss a breakthrough: Join the SciTechDaily newsletter.
Follow us on Google and Google News.