Patterns of Life Generated by Random Gene Pulsing in Cambridge Lab

Growing Biofilm Timelapse

Time-lapse over 60 hours showing the growing biofilm and the pattern development. Credit: University of Cambridge

A team of Cambridge scientists working on the intersection between biology and computation has found that random gene activity helps patterns form during the development of a model multicellular system.

We all start life as a single cell, which multiplies and develops into specialized cells that carry out different functions. This complex process relies on precise controls along the way, but these new findings suggest random processes also contribute to patterning.

In research published today (February 19, 2020) in Nature Communications, the scientists from James Locke’s team at the Sainsbury Laboratory Cambridge University and collaborators at Microsoft Research describe their discovery of surprising order in randomness while studying bacterial biofilms.

A biofilm develops when free-living single-celled bacteria attach to a surface and aggregate together to start multiplying and spreading across the surface. These multiplying individual cells mature to form a three-dimensional structure that acts like a multicellular organism.

And while individual cells can survive on their own, these bacteria prefer to work together with biofilms being the dominant form found in nature. The biofilm consortium provides bacteria with various survival advantages like increased resistance to environmental stresses.

Live Biofilm 12 Hour Intervals

Photos of the live biofilm taken at 12-hour intervals shows the development of the noisy gradient pattern in sigmaB expression over 24 hours. Credit: University of Cambridge

The researchers developed a new time-lapse microscopy technique to track how genetically identical single cells behave as the living biofilm developed.

Dr. Eugene Nadezhdin, joint lead-author, said: “We looked at how cells decide to take on particular roles in the biofilm. We found that towards the surface of the biofilm there were two different cell types frequently present — cells that form dormant spores and those that keep growing and activate protective stress responses. These two cell types are mutually exclusive, but they both could exist in the same location.”

They focussed on obtaining a detailed picture of how gene expression (whether genes are active or inactive) changes over time for the individual cell types, specifically on the expression of a regulatory factor, called sigmaB, which promotes stress responses and inhibits spore formation. They found that sigmaB randomly pulses on and off in cells at hourly intervals, generating a visible pattern of sporulating and stress-protected cells across the biofilm.

To understand the implications of the pulsing, the researchers generated a mathematical model of the sigmaB-controlled stress response and sporulation systems.

Timelapse Tracking Cells SigmaB Pulsing

Time-lapse tracking cells over 50 hours show sustained random SigmaB pulsing at the top of the biofilm. Credit: University of Cambridge

Dr. Niall Murphy, joint lead-author, said: “The modeling revealed that the random pulsing means that at any one time only a fraction of cells will have high sigmaB activity and activation of the stress pathway, allowing the remainder of cells to choose to develop spores. While the pulsing is random, we were able to show through a simple mathematical model that increasing expression of the gene creates shifting patterns among the different regions of the biofilm.”

The results demonstrate how random pulsing of gene expression can play a key role in establishing spatial structures during biofilm development.

Dr. Locke said: “This randomness appears to control the distribution of cell states within a population — in this case a biofilm. The insights gained from this work could be used to help engineer synthetic gene circuits for generating patterns in multi-cellular systems. Rather than the circuits needing a mechanism to control the fate of every cell individually, noise could be used to randomly distribute alternative tasks between neighboring cells.”

Reference: “Stochastic pulsing of gene expression enables the generation of spatial patterns in Bacillus subtilis biofilms” by Eugene Nadezhdin, Niall Murphy, Neil Dalchau, Andrew Phillips, and James C. W. Locke, 19 February 2020, Nature Communications.
DOI: 10.1038/s41467-020-14431-9

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