Biophysicists detail how they engineered synthetic materials that mimic the remarkable complexity of living organisms, successfully implementing a minimalistic model of the cell that can change its shape and move on its own.
Cells are complex objects with a sophisticated metabolic system. Their evolutionary ancestors, the primordial cells, were merely composed of a membrane and a few molecules. These were minimalistic yet perfectly functioning systems.
Thus, “back to the origins of the cell” became the motto of the group of TUM Professor Andreas Bausch, who is member of the cluster of excellence “Nanosystems Initiative Munich (NIM)” and his international partners. Their dream is to create a simple cell model with a specific function using a few basic ingredients. In this sense they are following the principle of synthetic biology in which individual cellular building blocks are assembled to create artificial biological systems with new characteristics.
The vision of the biophysicists was to create a cell-like model with a biomechanical function. It should be able to move and change its shape without external influences. They describe how they turned this goal into reality in the current edition of the academic journal Science, where their research is featured as cover story.
The magic ball
The biophysicists’ model comprises a membrane shell, two different kinds of biomolecules and some kind of fuel. The envelope, also known as a vesicle, is made of a double-layered lipid membrane, analogous of natural cell membranes. The scientists filled the vesicals with microtubules, tube-shaped components of the cytoskeleton, and kinesin molecules. In cells, kinesins normally function as molecular motors that transport cellular building blocks along the microtubules. In the experiment, these motors permanently push the tubules alongside each other. For this, kinesins require the energy carrier ATP, which was also available in the experimental setup.
From a physical perspective, the microtubules form a two-dimensional liquid crystal under the membrane, which is in a permanent state of motion. “One can picture the liquid crystal layer as tree logs drifting on the surface of a lake,” explains Felix Keber, lead author of the study. “When it becomes too congested, they line up in parallel but can still drift alongside each other.”
Decisive for the deformation of the artificial cell construction is that, even in its state of rest, the liquid crystal must always contain faults. Mathematicians explain these kinds of phenomena by way of the Poincaré-Hopf theorem, figuratively also referred to as the “hairy ball problem.” Just as one can’t comb a hairy ball flat without creating a cowlick, there will always be some microtubules that cannot lay flat against the membrane surface in a regular pattern. At certain locations the tubules will be oriented somewhat orthogonally to each other – in a very specific geometry. Since the microtubules in the case of the Munich researchers are in constant motion alongside each other due to the activity of the kinesin molecules, the faults also migrate. Amazingly, they do this in a very uniform and periodic manner, oscillating between two fixed orientations.
As long as the vesicle has a spherical shape, the faults have no influence on the external shape of the membrane. However, as soon as water is removed through osmosis, the vesicle starts to change in shape due to the movement within the membrane. As the vesicle loses ever more water, slack in the membrane forms into spiked extensions like those used by single cells for locomotion.
In this process, a fascinating variety of shapes and dynamics come to light. What seems random at first sight is, in fact, following the laws of physics. This is how the international scientists succeeded in deciphering a number of basic principles like the periodic behavior of the vesicles. These principles, in turn, serve as a basis for making predictions in other systems.
“With our synthetic biomolecular model we have created a novel option for developing minimal cell models,” explains Bausch. “It is ideally suited to increasing the complexity in a modular fashion in order to reconstruct cellular processes like cell migration or cell division in a controlled manner. That the artificially created system can be comprehensively described from a physical perspective gives us hope that in the next steps we will also be able to uncover the basic principles behind the manifold cell deformations.”
This research was supported in part by the German Excellence Initiative through the TUM Institute for Advanced Study and the Excellence Cluster Nanosystems Initiative Munich. The work of the Munich biophysicists was done in collaboration with colleagues from Brandeis University in Waltham, USA, the International School for Advanced Studies in Trieste, Italy and Syracuse University in New York, USA.
Publication: Felix C. Keber, et al., “Topology and dynamics of active nematic vesicles,” Science 5 September 2014: Vol. 345 no. 6201 pp. 1135-1139; DOI: 10.1126/science.1254784
Image: Keber, Loiseau, Sanchez, Bausch/TUM
half a ball slides around a Mobius strip and stays concave or convex,
in 3 space you can compress half the ball from convex to concave, so a jump occurs to the next 0/1 space up or down,(=1/2+)or (1/2 =1)= (3+space)the opposite is (3- space), the force +/- alters and3 space remains the same, the half ball having 1/2+ or – (potential-force-dimension) for change
(the quantum dimension = -half +half) and is virtual 1 or 0, see the golden rule for proof
should read, (half+)+(half-)=1, and 3D space remains the same,
the half balls potential changes, positive/negative
half plus, added to half minus = 1 potential, missed a sign off last note,
got my-self stuck in a loop !!!:)
a penny goes head to tails, a ball goes positive to minus spin on a Mobius-strip light goes in a edge/point circle at the event horizon, therefore time becomes static/stopped, in 3space we see an expansion, light never turns back to its source ,the expansion is a black-hole event-horizon becoming larger
I am I, the conjugate, the be all and end all, the chicken and the egg and =Q….. IF THAT HELPS, ….TIME is STATIC on an Event Horizon
PEACE @ SERINITY …. AS THE SONG GOES ”’ALL”’ ALL TOGETHER NOW’ 🙂
a clean sheet of paper, TABULA RASA…… seize the day DAY (CARPE DIEM),
a sheet of paper no matter how big comes down to crunch, are you getting THE POINT!, ALL LINES COVERGE, concave and convex are equal, we live in a body of matter 3D & have a 4D MIND, ID = Q e,pi e
ADD, to, graph theory, an exclamation ! mark is a point, with a line having only one TIME-direction, we view light coming in(PAST) not going out,(FUTURE) …. OUR FURTURE IS THE PAST WE observe, we LIVE in THE NOW-STATIC as observers of fixed-outer-limit light that is going to a point, therefore 0=1….BIG BANG…….BIG CRUNCHBIG BANG….BIG CRUNCH………………………..and on and on and on and on A NOBIUS STRIP ,FROM APE TO ‘HUMAN BEING’!!! to ”’Q”’
to get off the strip is to become (ID,Q) which = ”’O”’
I AM TRYING TO MAKE YOU (ID,Q) then we ALL can go to the NEXT-STEP
the previous should have read e,pi,i….., concentration-skew-within 3D/4D
3 INFINITES AND A THIRD OF EACH =4D therefore ABRACADABRA, OPEN-SESAME,
THE CAT IS OUT OF THE BOX, GOD does not plague the COSMOS with mice 🙂
if I have a huge-ball cut in half and mirror the inside and then step,inside with my cat and an -off miners-light, strapped around my forehead, THE SHERE THEN SEALED.
who will let the cat out for ME while I am QUANTUM TUNNELING?
THE LIGHT IS NOW ON……….WOW!!! OR WOW!!! 4D!!!!