CRISPR’s Potential and Dangers: Is CRISPR Worth the Risk?

Is CRISPR Worth the Risk

The gene editing technology CRISPR has prompted both breathless predictions of medical breakthroughs and warnings of apocalypse. Yale Insights asked Dr. Greg Licholai, a biotech entrepreneur and a lecturer at Yale SOM, to explain CRISPR’s potential and dangers.

Recently, HBO’s John Oliver opened a Last Week Tonight segment with a series of video clips about gene editing—some of them news reports promising amazing breakthroughs, others movie scenes depicting genetic engineering gone terribly wrong.

“It seems gene editing is going to eliminate all disease,” he concluded. “Or kill every last one of us.”

For decades, advances in generic engineering have prompted both breathless predictions of a wondrous future and warnings of apocalypse. Both have gotten louder in the five years since the development of CRISPR, which allows for much more precise editing of genes than previously existing tools.

In 2017, for the first time, scientists used CRISPR to repair a genetic mutation—one that could cause a heart defect—in an embryo. Reporting the breakthrough, the New York Times said that “it raises the prospect that gene editing may one day protect babies from a variety of hereditary conditions.” But in the article’s third paragraph, the newspaper added that the successful experiment “is sure to renew ethical concerns that some might try to design babies with certain traits, like greater intelligence or athleticism.”

In the last few months, more immediate concerns have arisen about CRISPR. A series of studies have suggested that CRISPR may cause cells to lose their cancer-fighting ability, and that it may do more damage to genes than previously understood. “It is important that anyone thinking of using this technology for gene therapy proceeds with caution, and looks very carefully to check for possible harmful effects,” said researcher Allan Bradley in a release from the Wellcome Sanger Institute.

Do CRISPR’s benefits outweigh the risks? Yale Insights asked Dr. Gregory Licholai, a biotech entrepreneur who serves as a lecturer at Yale SOM and chief medical and information officer at PRA Health Sciences, to explain the technology’s potential and dangers.

What is CRISPR and how is it different from the methods that have been used to manipulate genetics before?

CRISPR is this fascinating, powerful technology. It’s got a very clunky name. It’s called Clustered Regularly-Interspaced Short Palindromic Repeats. Now what does that mean? The name actually refers to the way it interacts with DNA. It’s a way to manipulate DNA, to edit DNA, in a way that is much more powerful than previous methods, much simpler, much cheaper. And the important part is it’s exceptionally precise.

So as you probably know, our book of life is made of DNA. DNA itself is many millions of base-pairs, which is like a language. And within that language, there are certain regions which code for genes, and those genes are incredibly important because those genes go on to make up everything about us. There’s 40,000 proteins that become outputs of those genes and they are involved in our health, our wellbeing, and any defect in those genes becomes problematic and causes disease.

What was previously attempted with gene editing was to manipulate genetic information in blocks, basically in big pieces. It’s kind of like trying to edit a book by only being able to rip out a page at a time and transfer a page at a time, without really being able to control the actual words. The power of this technology: it literally comes down to the individual letters. So the precision is far better than anything that has happened before. The excitement in the scientific community is being able to go in and very precisely make changes in DNA of actual genes that you can actually turn off bad genes or you can potentially repair genes that have got mutations in them where the code is written incorrectly.

There had been previous gene-editing technologies, such as viral gene editing, gene replacement, and those have developed over several decades. One of the most fascinating things about CRISPR is how quickly everything is developed, so in less than 10 years since the initial descriptions and initial papers were written, this technology has just exploded. It’s been less than five years since the initial patents were written, and since then at least a half-dozen companies have been formed, all of them are racing forward to try to get a leg up on each other to try to proceed with using CRISPR for various applications.

What are some of the applications, in the somewhat reasonable, predictable future?

There are three main applications for CRISPR. One is in manipulating genes to turn them on or off within people. Another is to create medications that can be infused, or in some cases, self-therapy—taking blood and certain cells out of a body, manipulating them with CRISPR, and then putting them back in. The third, which sometimes is overlooked, is actually in farming. Both farming with animals as well as farming with crops. And in fact, the application of CRISPR to foods has already been done. There are companies that have already been using CRISPR to create enhanced foods to resist bacteria or viruses. To create even better-tasting foods. And that’s already being done.

Similarly, the application of CRISPR to animals has already been done. They actually call them CRISPR mice, and they are already being used in the research community. The ability to apply it to larger animals such as food animals is in the very near future.

In terms of human health, we can divide that into two different categories. One is taking cells out of the body, manipulating them in the laboratory—either removing a defective gene or adding and enhancing an ability to do something by turning on a gene or fixing a gene—and then putting those cells back in the body. That’s one category. The other category would be actually injecting something into the body which can edit people’s genes so that within their own tissues those genes can either be turned on or off. And all of these have got some pretty profound complications and risks.

Can you give an example of a disease that could be treated?

There are over 7,000 monogenetic diseases that we can trace back to a single gene that has a defect. Often those genes have multiple mutations. But at least a single gene has been identified. The most promising application of CRISPR would be to modify those monogenetic diseases. And those monogenetic diseases, they broadly fall into two categories, curiously named toxic gain of function and toxic loss of function.

Toxic loss of function is kind of intuitive. That means the gene has got a defect in it; the person loses the function of that protein and that causes the disease. So a well-known, well-studied example would be sickle-cell anemia. A single base-pair mutation actually causes a change in the structure of hemoglobin that then creates this unique sickle-cell shape for red blood cells.

An example of toxic gain of function is disease called transthyretin in which a mutation causes a clumping up of different proteins. And that leads to a disease called amyloidosis, where these proteins, which normally don’t stick together, because of this kink in them due to the mutation, they become very sticky. They form aggregates and those aggregates can build up in various cells in the body. Sometimes the brain, sometimes the heart.

CRISPR could potentially be useful in either one of those, and in fact there are companies that are looking at those diseases, as well as a number of others. Other monogenetic diseases would be cystic fibrosis, beta thalassemia, glycogen storage disease, Behçet’s disease, Fabry disease. Some of these are quite rare, like Fabry disease, but some are more common, like cystic fibrosis, which is the most common genetic disease in Caucasians.

So genetic diseases is one category. Another category is oncology. It’s been well known now for some years that our own immune system has the ability to fight cancer cells and essentially dissolve micro-tumors. But cancer is a clever entity—it evades the body’s internal immune system. So what that means is that the cancer becomes invisible to our immune cells, and that invisibility is due to certain proteins that are created as checkpoints to interfere with the immune system attacking ourselves. Cancers, essentially, mimic our own cells by taking advantage of these checkpoints.

So one of the applications of CRISPR would be to remove immune cells from the body, apply the CRISPR technology, and then turn off these checkpoints and put those immune cells back in the body with the hope that then those immune cells would clear the tumor away.

What are the risks of this? Are the risks to the patient? Or to all of us?

One of the biggest risks of CRISPR is what’s called gene drive, or genetic drive. What that means is that because you’re actually manipulating genes and those genes get incorporated into the genome, into the encyclopedia, basically, that sits within cells, potentially those genes can then be transferred on to other organisms. And once they’re transferred on to other organisms, once they become part of the cycle, then those genes are in the environment.

That’s probably the biggest fear of CRISPR. Humans manipulating the genetic code, and those manipulations get passed on generation to generation to generation. We think we know what we’re doing, we think we’re measuring exactly what changes we’re doing to the genes, but there’s always the possibility that either we miss something or our technology can’t pick up on other changes that have been made that haven’t been directed by us. And the fear then is that those changes lead to antibiotic resistance or other mutations that go out into the population and would be very difficult to control. Basically creating incurable diseases or other potential mutations that we wouldn’t really have control over.

Is there consensus on how to proceed?

There’s been discussion in the scientific community in the United States and globally about how to proceed with CRISPR. And particularly some very high-placed scientists, in the United States, for example, the former director of the National Institute of Health, have called for a self-imposed ethical moratorium on CRISPR until more is known. Not on all types of CRISPR research, but for certain types of CRISPR research. Particularly on these germline mutations that could potentially be passed on through generations. Some of the inventors of the patent-holders of CRISPR technologies who are now the inventors of the various companies in biotechnology, they’ve also imposed their own moratoria on working in germ lines until more is understood. So there are parts of the scientific community that are very concerned and are trying to be very thoughtful about how to proceed and how to proceed safely.

In the United States, there have been some regulations against moving forward in areas that aren’t safely understood. That doesn’t exist in other parts of the world, in particular in China. So there’s been several examples now of where China has leaped ahead of what’s going on in Europe or the United States but, the concern is, without the kind of regulatory and ethical safeguards that are in place in other countries.

You mentioned that there’s, in the U.S. at least, there’s a moratorium on germline mutations. Do the kinds of treatments that you’ve talked about before, do those require the germline modification, or can they be done within the stricture of that moratorium?

No, the treatment of most of those diseases, monogenetic diseases—things like cystic fibrosis, sickle-cell, beta thalassemia—those are not germline mutations. So theoretically, it would be safe to be able to treat those patients without the theoretical concern of affecting germ lines and affecting gene drive. The same thing with oncology. Theoretically you’re just taking cells out. You’re only treating immune cells and they’re not going replicate. On the other hand, as soon as people start talking about stem cells and then manipulating stem cells and then reusing those, then those stem cells can potentially affect other cells that replicate. The risk is low, but there’s definitely a risk there.

One of the other places that this is being actively worked on is, again, in animals. The idea would be to introduce mutations into, say, malaria-bearing mosquitoes, and let them in the wild and eradicate mosquitoes. Or eradicate certain types of invasive plants by introducing some kind of genetic manipulation that gets passed on and, again, you take out that one particular species. Again, it raises concerns. We think we know what we’re affecting if we manipulate one gene for that particular species. We think we know what we’re affecting if we just affect one particular species in an ecosystem. The truth is we probably don’t, and there’s always some surprises.

You mentioned that Chinese researchers are operating in different structure. Can you expand on that, on what regulations they have and what that means in terms of their competition with companies in the U.S.?

One of the dramatic examples happened in 2016. There had been a self-imposed moratorium in the United States and Europe to work on germ cells, germ lines. And that would include human embryos. Now, at the same time, reports came out of China that researchers had begun working on human embryos. Initially in 2015 and ’16, the reports were that the experiments were negative, and at least the Chinese researchers had claimed that they were working with nonviable human embryos anyway. In the short time since then, in the year and a half since then, those experiments have been repeated, apparently with scientific success, whatever that means. But without the kind of self-imposed regulation or even organizationally imposed regulation that we would have by the NIH or the scientific community in the United States and Europe.

Another example is that researchers in China have actually proceeded to human clinical trials using CRISPR much faster than has been possible in the United States. Normally, the clinical trial process to test any new therapy requires several very well studied stages. The first stage is to test in animals to make sure that there’s complete safety. Then it goes into very limited testing in human beings, just for safety, and then proceeds from there. Apparently in China, they took the animal data and they went right into therapeutic trials in human beings. And the most recent reports that are that somewhere between 80 and 100 people are already being tried, or already being tested using CRISPR.

We know that in China, they’re using CRISPR for cancer therapy. That’s the example where cells are taken out of the body, their immune cells are manipulated with CRISPR and then they’re re-infused. It’s too early to tell if it’s successful or not. But there is a lot of concern that the regulatory authorities in China have been extremely permissive with allowing these technologies to move forward. And that has a lot of profound implications.

How fast is this technology changing? Given that it’s moved as far as it has in 10 years, where do you expect it to move in another 5 or 10 years?

It’s changing pretty fast. Just in the last few months, there’s new developments in the field in CRISPR. Some is around competition, with new companies being formed. In fact, one of the original developers of CRISPR science that comes out of the Broad Institute at Harvard/MIT just set up a new company. Another scientific development is that there’s now scientific evidence that perhaps in some people, they have naturally occurring immunity, if you will, to CRISPR. They have naturally occurring substances that actually will turn off any kind of CRISPR that’s put into them.

All of these things are brand new, and they’re all being sorted out by scientific community, by these biotech companies. So it is changing very quickly. And the other thing that’s changing is the effect of this international competition. I don’t think anybody could have predicted that other countries, and China in particular, would be so quick to embrace this technology and really leap forward ahead of everybody else.

Are there any other safety concerns with CRISPR?

As with any new technology, there could be scientific bumps in the road. The safety concern is that in this field is moving so quickly and some researchers want to get into human clinical trials right away, even before the CRISPR technology paradigm has been fully validated. There are some recent reports in the scientific literature that this approach is not as precise as advertised. In other words, we think we are editing one letter of the book of life, but it actually entire pages might be getting altered in unintended areas. The long-term danger is unintended changes to the genome of an organism that go on and get carried through to the next generation. The safety risk is unknown changes in genes that get transferred to the population that could have no consequence or could be harmful.

How can we ensure that the field progresses in a safe way?

The drug development process is tightly regulated across the world. In the United States the FDA closely monitors safety of any investigational drug, and all CRISPR drugs intended to go into people would have to meet the same rigorous testing standards. The same situation exists for Europe and the rest of the world where regulatory authorities largely work in harmony. However, there are exceptions, as with some of the human embryo testing that has been reported in China. We should make sure that the level of international scientific regulation and cooperation continues so the scientific developments can continue but also ensure safety.

5 Comments on "CRISPR’s Potential and Dangers: Is CRISPR Worth the Risk?"

  1. “…basically creating incurable diseases that we wouldn’t have any control over.”

    That’s how this video ends.

    Enter Capitalism, and then add-in a company’s potential for making a buttload of $$ to the equation. Then this will become the most likely outcome of this seeming miraculous technology.

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  3. Dustin Graybill | October 3, 2018 at 8:23 am | Reply

    …basically creating incurable diseases that we wouldn’t have any control over.”

    That is, at least, until we CRISPR them again to make them curable and controllable.

    We have the ability to plan our building blocks. Like any building there will be failures that we will learn from and establish building codes for future development. The real Doomsday and Risk is leaving our Genetics up to random mutations and mate selection. It would be irresponsible and negligent of our human race to actively stay ignorant to improving our genetic code.

    We are more at risk of a future closer to that of “Idiocracy” without using CRISPR. This is where ignorance propagates more ignorance and begins a devolution of humanity.

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