University of Queensland researchers used real-time imaging on quail embryos to study heart and neural development, providing potential breakthroughs in understanding congenital defects.
Researchers at The University of Queensland have for the first time captured images and video in real time of early embryonic development to understand more about congenital birth defects.
Dr. Melanie White and Dr. Yanina Alvarez from UQ’s Institute for Molecular Bioscience used quail eggs to understand how cells begin to form tissues such as the heart, brain, and spinal cord.
Dr. White said congenital birth defects affect 3 percent of Australian babies with heart defects the most common and neural tube defects second.
“Because quails grow in an egg, they’re very accessible for imaging and their early development is very similar to a human at the time the embryo implants in the uterus,” Dr. White said.
“For the first time, we have seen high-resolution, real-time imaging of important early developmental processes.
“Until now, most of our knowledge of post-implantation development came from studies on static slides, at fixed points in time.”
Studying Cellular Dynamics With Fluorescent Proteins
The IMB researchers have generated quails with a fluorescent protein to reveal the structure, called the actin cytoskeleton, which gives cells shape and facilitates movement.
“When cells migrate during early development, they stick out protrusions called lamellipodia and filopodia-like arms that reach out and grab onto surfaces allowing the cells to crawl, or reach other cells to bring them closer together,” Dr. White said.
Visualizing Heart and Neural Development
“We were able to image the filopodia from heart stem cells deep inside the embryo as they first made contact by sticking out protrusions and gripping to their surroundings and each other to form the early heart.
“It’s the first time anyone has captured the cell’s actin cytoskeleton facilitating this contact in live imaging.”
The researchers also imaged the open edges of the neural tube and it being ‘zipped up’ to begin to form the brain and spinal cord.
“We saw how the cells reached across the open neural tube with their protrusions to contact the opposite side — the more protrusions the cells formed, the faster the tube zipped up,” Dr White said.
Potential Implications for Congenital Defect Research
“If this process goes awry or is disrupted and the tube doesn’t close properly during the fourth week of human development, the embryo will have brain and spinal cord defects.
“Our aim is to find proteins or genes that can be targeted in the future or used for screening for congenital birth defects.
“We are very excited at the possibilities that this new quail model now offers to study development in real-time.”
Reference: “A Lifeact-EGFP quail for studying actin dynamics in vivo” by Yanina D. Alvarez, Marise van der Spuy, Jian Xiong Wang, Ivar Noordstra, Siew Zhuan Tan, Murron Carroll, Alpha S. Yap, Olivier Serralbo and Melanie D. White, 24 June 2024, Journal of Cell Biology.
DOI: 10.1083/jcb.202404066
The research was published in the Journal of Cell Biology by a team that included Marise van der Spuy and Jian Xiong Wang from UQ’s Institute for Molecular Bioscience.
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