New Research: A Single Sleepless Night Can Rapidly Reverse Depression for Days

Depression Relief Concept

Researchers at Northwestern University found that acute sleep deprivation enhances dopamine release and brain plasticity, causing a temporary uplifted mood and offering insights into potential new targets for antidepressants.

A new study has found that acute sleep loss leads to an increase in dopamine release and rewires the brain.

Most individuals who have experienced staying up all night know the peculiar sensation of being both fatigued and hyperactive. Despite physical weariness, the brain feels slap-happy, loopy, and almost giddy.

Now, Northwestern University neurobiologists are the first to uncover what produces this punch-drunk effect. In a new study, researchers induced mild, acute sleep deprivation in mice and then examined their behaviors and brain activity. Not only did dopamine release increase during the acute sleep loss period, synaptic plasticity also was enhanced — literally rewiring the brain to maintain the bubbly mood for the next few days.

These new findings could help researchers better understand how mood states transition naturally. It also could lead to a more complete understanding of how fast-acting antidepressants (like ketamine) work and help researchers identify previously unknown targets for new antidepressant medications.

The research was recently published in the journal Neuron. Northwestern postdoctoral fellow Mingzheng Wu is the paper’s first author, and Professor Yevgenia Kozorovitskiy is the corresponding author.

“Chronic sleep loss is well studied, and its uniformly detrimental effects are widely documented,” Kozorovitskiy said. “But brief sleep loss — like the equivalent of a student pulling an all-nighter before an exam — is less understood. We found that sleep loss induces a potent antidepressant effect and rewires the brain. This is an important reminder of how our casual activities, such as a sleepless night, can fundamentally alter the brain in as little as a few hours.”

An expert in neuroplasticity, Kozorovitskiy is an associate professor of neurobiology and the Irving M. Klotz Professor at Northwestern’s Weinberg College of Arts and Sciences.

Signs of sleep loss

Scientists long have known that acute perturbations in sleep are associated with altered mental states and behaviors. Alterations of sleep and circadian rhythms in patients, for example, can trigger mania or occasionally reverse depressive episodes.

“Interestingly, changes in mood state after acute sleep loss feel so real, even in healthy subjects, as experienced by myself and many others,” Wu said. “But the exact mechanisms in the brain that lead to these effects have remained poorly understood.”

To explore these mechanisms, Kozorovitskiy and her team developed a new experiment to induce acute sleep loss in mice that did not have genetic predispositions related to human mood disorders. The experimental setup needed to be gentle enough to avoid causing substantial stress for the animals but just uncomfortable enough to prevent the animals from falling asleep. After a sleepless night, the animals’ behavior shifted to become more aggressive, hyperactive, and hypersexual, compared to controls that experienced a typical night’s sleep.

Using optical and genetically encoded tools, the researchers measured the activity of dopamine neurons, which are responsible for the brain’s reward response. And they found activity was higher in animals during the brief sleep loss period.

“We were curious which specific regions of the brain were responsible for the behavioral changes,” Kozorovitskiy said. “We wanted to know if it was a large, broadcast signal that affected the entire brain or if it was something more specialized.”

Specialized signal

Kozorovitskiy and her team examined four regions of the brain responsible for dopamine release: the prefrontal cortex, nucleus accumbens, hypothalamus, and dorsal striatum. After monitoring these areas for dopamine release following acute sleep loss, the researchers discovered that three of the four areas (the prefrontal cortex, nucleus accumbens, and hypothalamus) were involved.

But the team wanted to narrow down the results even further, so they systematically silenced the dopamine reactions. The antidepressant effect disappeared only when researchers silenced the dopamine response in the medial prefrontal cortex. By contrast, the nucleus accumbens and hypothalamus appeared to be most involved in the hyperactivity behaviors but were less connected to the antidepressant effect.

“The antidepressant effect persisted except when we silenced dopamine inputs in the prefrontal cortex,” Kozorovitskiy said. “That means the prefrontal cortex is a clinically relevant area when searching for therapeutic targets. But it also reinforces the idea that has been building in the field recently: Dopamine neurons play very important but very different roles in the brain. They are not just this monolithic population that simply predicts rewards.”

Heightened neuroplasticity

While most of the behaviors (such as hyperactivity and increased sexuality) disappeared within a few hours following acute sleep loss, the antidepressant effect lingered for a few days. This suggested that synaptic plasticity in the prefrontal cortex might be enhanced.

When Kozorovitskiy and her team examined individual neurons, they discovered just that. The neurons in the prefrontal cortex formed tiny protrusions called dendritic spines, highly plastic features that change in response to brain activity. When the researchers used a genetically encoded tool to disassemble the synapses, it reversed the antidepressant effect.

Evolving to avoid predators?

While researchers do not fully understand why sleep loss causes this effect in the brain, Kozorovitskiy suspects evolution is at play.

“It’s clear that acute sleep deprivation is somehow activating to an organism,” Kozorovitskiy said. “You can imagine certain situations where there is a predator or some sort of danger where you need a combination of relatively high function with an ability to delay sleep. I think this could be something that we’re seeing here. If you are losing sleep routinely, then different chronic effects set in that will be uniformly detrimental. But in a transient way, you can imagine situations where it’s beneficial to be intensely alert for a period of time.”

Kozorovitskiy also cautions people not to start pulling all-nighters in order to brighten a blue mood.

“The antidepressant effect is transient, and we know the importance of a good night’s sleep,” she said. “I would say you are better off hitting the gym or going for a nice walk. This new knowledge is more important when it comes to matching a person with the right antidepressant.”

Reference: “Dopamine pathways mediating affective state transitions after sleep loss” by Mingzheng Wu, Xin Zhang, Sihan Feng, Sara N. Freda, Pushpa Kumari, Vasin Dumrongprechachan and Yevgenia Kozorovitskiy, 2 November 2023, Neuron.
DOI: 10.1016/j.neuron.2023.10.002

The study was supported by the One Mind Nick LeDeit Rising Star Research Award, the BD2: Breakthrough Disocoveries for Thriving with Bipolar Disorder (Discovery Award), the National Institutes of Health (grant numbers R01NS107539 and R01MH117111) and the Rita Allen Foundation Scholar Award. Some of the co-authors were supported by fellowship awards, including the Christina Enroth-Cugell and David Cugell Fellowship, the American Heart Association, and NIH training programs (award numbers T32AG20506 and 2T32GM15538). 

3 Comments on "New Research: A Single Sleepless Night Can Rapidly Reverse Depression for Days"

  1. Kuldeep Kumar Kaul | December 28, 2023 at 9:01 pm | Reply

    Kozorovitskiy and her team may like to study the effect of My Sleeping only 4 hrs from 11.00 p.m to 3.00 a.m. since last one decade.
    At the age of 74 I feel fully fit and active throughout rest of 20 hrs!

    Here under I am reproducing my answer to a Question appeared on QURA Forum:

    Why do yogis say 4 hours of sleep is enough? by Kuldeep Kaul

    Why do yogis say 4 hours of sleep is enough?
    Scientifically, it is found that one full cycle of sleep is of four hours only. But, after completing one cycle, due to one’s laziness he remains in lying position and goes into second cycle of sleep of next four hours. Thus, mistakingly, even medical science recommend minimum of 8 hours sleep!

    The reason for this laziness is because, the cycle time of four hours sleep is not being commonly selected as the most appropriate one which, is from 11 p.m. to 3 a.m. From scientific research, it is found that during these four hours maximum blood remains in liver, while rest of the body remains with less than average blood normally body contains. It is because of that reason body , brain in particular needs full rest. Therefore, it is essential to have full rest of the body so that liver will function in best possible manner. To get both the functions satisfactorily performed viz., rest to brain n body and best liver working, one should sleep from 11 p.m. to 3 a.m.without any disturbances. If this cycle is well maintained then only, one cycle of sleep is sufficient for living a normal day to day life during the day.

    In the light of above fact, probably Yogis were aware of this and must have found it by hit and trial to fix 11 p.m. to 3 a.m. for sleep. After 3 a.m. blood starts going, in excess quantity, to lungs which, remains there in excess upto 5.00 a.m. . That is why Yogis devot this time of 3 a.m. to 5 a.m. for Pranayamas, Yogasanas and Meditation etc.

  2. Sanette Shirley | January 1, 2024 at 1:18 am | Reply

    Great research!

Leave a comment

Email address is optional. If provided, your email will not be published or shared.